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Оптимизация методов криоконсервации гермоплазмы чёрной смородины (Ribes nigrum l.)
УДК:
581.1.083: 634.7.723
Автор статьи:
Ковальчук И.Ю.
Соавторы:
Турдиев Т. Т.
Место работы автора:
Институт биологии и биотехнологии растений Национального центра биотехнологииРеспублики Казахстан КН МОН РК
Название журнала:
Биотехнология. Теория и практика
Год выпуска:
2010
Номер журнала:
2
Страницы:
с.54-61
Резюме на казахском языке:
Қара қарақаттың оқшау алған ұлпаларын сұйық азотта криоконсервілеу ?дістері үйлестірілді. Апикальды меристеманы суыққа бейімдеудің оңтайландырылған уақыты 3-5 апта аралығы.Тұрақты төменгі (+4?С) температураға қарағанда ауыспалы температурада (8 час +22?С / 16 час -1?С) суыққа бейімдеу тиімді. 0,3М сахароза қосылған витрификация ?дісімен ж?не 2Мглицеринмен өңделген 0,4М сахароза бар МС қоректік ортада криоконсервілеу ?дісі ұтымды болып көрінді. Өсімдік ұлпаларын криоконсервілеу үшін PVS2 криопротекторымен өңдеу ең тиімді болды. Қара қарақаттың 11 сортының ж?не будандарының бұтақшаларының апикальды меристемалары сұйық азот құйылған дьюарда ұзақ уақыт сақтауға қойылды.
Резюме на английском языке:
The methods of cryopreservation of isolated black currant tissue in liquid nitrogen were optimized. Optimal duration of apical meristems cold acclimation was 3-5 weeks. Acclimation by alternatingtemperatures (8 h at +22?C / 16 h at -1?C) was more effective than constant temperature (+4?C). The optimal pretreatment for vitrifi cation method was preculture of meristem on medium with 0.3M sucroseand treatment by 2M glycerol. The cryoprotectant PVS2 was the best for cryopreservation. The apical meristems of 11 cultivars and hybrids of black currant were placed in Dewar vessels with liquid nitrogen for long-term cryopreservation.
Список литературы:
1. Chang Y, Reed B M Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. CryoLetters. – 1999 . – 20. – P. 371-376.
2. Reed B.M. Cryopreservation of Temperate Berry Crops / Reed B.M. (Ed) // Plant Cryopreservation: A Practical Guide. – New York: Springer Science + Business Media LLC, 2008. – P. 333-364.
3. Engelmann F., Gonzalez Arnao M.T, Wu Y, Escobar R.H. Development of Encapsulation Dehydration / Reed B.M. (Ed) // Plant Cryopreservation: A Practical Guide. – New York: Springer Science + Business Media LLC. – 2008. – P. 59-76.
4. Matsumoto T., Sakai A. Cryopreservation of grape in vitro-cultured axillary shoot tips by threestep vitrifi cation / Engelmann F., Takagi H. (Eds) // Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Application. – Rome: Japan International Research Center for Agricultural Sciences and International Plant Genetic Resources Institute. – 2000. – Р.424- 425.
5. Luo J., Reed B.M. Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrifi cation // Cryobiology. – 1997. – N 34. – P. 240-250.
6. Dereuddre J., Scottez C., Arnaud Y., Duron M. Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv. Beurre Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen // C. R. Acad. Sci. Paris. – 1990. – N 310 – P. 317-323.
7. Reed B. M. Implementing cryogenic storage of clonally propagated plants // CryoLetters. – 2001. – N 22. – P. 97-104.
8. Chang Y, Reed B M Effects of photoperiod and alternating temperature on the cryopreservation and cold hardiness of in vitro-grown Pyrus meristems. In Vitro Cellular and Developmental Biology. – 1998. – P. 34-61.
9. Chang Y, Reed B M Preculture conditions infl uence cold hardiness and regrowth of Pyrus cordata shoot tips after cryopreservation. HortScience – 2001. – N 36. – P. 1329-1333.
10. Senula A, Keller E R J, Sanduijav T, Yohannes T Cryopreservation of cold acclimated mint explants using a simple vitrifi cation protocol. CryoLetters. - 2007. - N28. – P. 1-12.
11. Palonen P, Junttila O Cold hardening of raspberry plants in vitro is enhanced by increasing sucrose in the culture medium. Physiologia Plantarum. - 1999. - 106. – P. 386-392.
12. Reed BM Plant Cryopreservation: A Practical Guide. Springer Science + Business Media LLC, New York. – 2008. – P. 513.
13. Reed B. M. Implementing cryogenic storage of clonally propagated plants // CryoLetters/ – 2001. – N 22. – P. 97-104.
14. Dussert S, Engelmann F, Noirot M Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. CryoLetters – 2003. – N24. – P. 149- 160.
15. Wang Q, Laamanen J, Uosukainen M, Valkonen JPT Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrifi cation and encapsulation-dehydration. Plant Cell Reports . – 2005. – N24. – P. 280-288.
16. Gupta S, Reed BM Cryopreservation of shoot tips of blackberry and raspberry by encapsulationdehydration and vitrifi cation. CryoLetters. – 2006. – N27. – P. 29-42.
17. Hirai D., Sakai A. Cryopreservation of in vitro grown meristems of potato by encapsulationvitrifi cation // Cryopreservation of tropical plant germplasm. Current research progress and application. Eds. Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. – P. 205-211.
18. Golmirzaie A.M., Panta A. Advances in potato cryopreservation at CIP // Cryopreservation of tropical plant germplasm. Current research progress and application. Eds. Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. - P. 205-211.
19. Towill L.E. Cryopreservation of isolated mint shoot tips by vitrifi cation // Plant Cell Reports. – 1990.– N9. – P. 178-180.
20. Takagi H. Recent development in cryopreservation of shoots apices of tropical species //Cryopreservation of tropical plant germplasm. Current research progress and application. Eds.Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. – P. 178-193.
1. Chang Y, Reed B M Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. CryoLetters. – 1999 . – 20. – P. 371-376.
2. Reed B.M. Cryopreservation of Temperate Berry Crops / Reed B.M. (Ed) // Plant Cryopreservation: A Practical Guide. – New York: Springer Science + Business Media LLC, 2008. – P. 333-364.
3. Engelmann F., Gonzalez Arnao M.T, Wu Y, Escobar R.H. Development of Encapsulation Dehydration / Reed B.M. (Ed) // Plant Cryopreservation: A Practical Guide. – New York: Springer Science + Business Media LLC. – 2008. – P. 59-76.
4. Matsumoto T., Sakai A. Cryopreservation of grape in vitro-cultured axillary shoot tips by threestep vitrifi cation / Engelmann F., Takagi H. (Eds) // Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Application. – Rome: Japan International Research Center for Agricultural Sciences and International Plant Genetic Resources Institute. – 2000. – Р.424- 425.
5. Luo J., Reed B.M. Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrifi cation // Cryobiology. – 1997. – N 34. – P. 240-250.
6. Dereuddre J., Scottez C., Arnaud Y., Duron M. Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv. Beurre Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen // C. R. Acad. Sci. Paris. – 1990. – N 310 – P. 317-323.
7. Reed B. M. Implementing cryogenic storage of clonally propagated plants // CryoLetters. – 2001. – N 22. – P. 97-104.
8. Chang Y, Reed B M Effects of photoperiod and alternating temperature on the cryopreservation and cold hardiness of in vitro-grown Pyrus meristems. In Vitro Cellular and Developmental Biology. – 1998. – P. 34-61.
9. Chang Y, Reed B M Preculture conditions infl uence cold hardiness and regrowth of Pyrus cordata shoot tips after cryopreservation. HortScience – 2001. – N 36. – P. 1329-1333.
10. Senula A, Keller E R J, Sanduijav T, Yohannes T Cryopreservation of cold acclimated mint explants using a simple vitrifi cation protocol. CryoLetters. - 2007. - N28. – P. 1-12.
11. Palonen P, Junttila O Cold hardening of raspberry plants in vitro is enhanced by increasing sucrose in the culture medium. Physiologia Plantarum. - 1999. - 106. – P. 386-392.
12. Reed BM Plant Cryopreservation: A Practical Guide. Springer Science + Business Media LLC, New York. – 2008. – P. 513.
13. Reed B. M. Implementing cryogenic storage of clonally propagated plants // CryoLetters/ – 2001. – N 22. – P. 97-104.
14. Dussert S, Engelmann F, Noirot M Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. CryoLetters – 2003. – N24. – P. 149- 160.
15. Wang Q, Laamanen J, Uosukainen M, Valkonen JPT Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrifi cation and encapsulation-dehydration. Plant Cell Reports . – 2005. – N24. – P. 280-288.
16. Gupta S, Reed BM Cryopreservation of shoot tips of blackberry and raspberry by encapsulationdehydration and vitrifi cation. CryoLetters. – 2006. – N27. – P. 29-42.
17. Hirai D., Sakai A. Cryopreservation of in vitro grown meristems of potato by encapsulationvitrifi cation // Cryopreservation of tropical plant germplasm. Current research progress and application. Eds. Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. – P. 205-211.
18. Golmirzaie A.M., Panta A. Advances in potato cryopreservation at CIP // Cryopreservation of tropical plant germplasm. Current research progress and application. Eds. Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. - P. 205-211.
19. Towill L.E. Cryopreservation of isolated mint shoot tips by vitrifi cation // Plant Cell Reports. – 1990.– N9. – P. 178-180.
20. Takagi H. Recent development in cryopreservation of shoots apices of tropical species //Cryopreservation of tropical plant germplasm. Current research progress and application. Eds.Engelmann F& Takagi H., JIRCAS, Tsukuba & IPGRI, Rome. – 2000. – P. 178-193.
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Оптимизированы методы криоконсервации изолированных тканей чёрной смородины в жидком азоте. Оптимальная продолжительность холодовой акклиматизации для закаливания апикальных меристем составляла 3-5 недель. Акклиматизация при переменных температурах (8 час +22?С/16 час -1?С) былаболее эффективна, чем при постоянной температуре +4?С. Показано, что оптимальной предобработкойпри использовании метода витрификации является культивирование меристем на среде с 0,3М сахарозой и обработка 2М глицерином. Лучшим криопротектором для предобработки является PVS2. На длительное криосохранение в дьюары с жидким азотом помещены апикальные меристемы 11 сортов и гибридов чёрнойсмородины.
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