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Егеуқұйрықтың тері асты шандырларынан бөлініп алынғанLYVE-1+макрофагтардың функционалдық белсенділігі
УДК:
576.3:599.323.4:591.44
Автор статьи:
Кумашева В.Т.
Соавторы:
Даңлыбаева Ғ.А., Огай В.Б.
Место работы автора:
Л.Н. Гумилев атындағы Еуразия ұлттық университеті
Название журнала:
Биотехнология. Теория и практика
Год выпуска:
2010
Номер журнала:
1
Страницы:
с.77-83
Резюме на казахском языке:
В данной работе изучали функциональную активность LYVE-1+макрофагов, выделенных из подкожной фасции крыс. Для определения функциональной активности мы использовалииммунофлуоресцентный анализ и цитотоксический тест для определения ФНО-альфа. Было обнаружено, что LYVE-1+макрофаги способны связывать гиалуроновую кислоту и активно еепоглощать. Кроме того, было показано, что ФНО продуцирующая активность LYVE-1+макрофагов, была значительно выше по сравнению с перитонеальными макрофагами крыс. Таким образом,основываясь на наших и литературных данных, мы предполагаем, что LYVE-1+макрофаги могут участвовать в гомеостазе метаболизма гиалуроновой кислоты в подкожной фасции, котораялишена лимфатических сосудов.
Резюме на английском языке:
In this work the function of activity of LYVE-1+macrophages isolated from the normal subcutaneous fascia of rats was studied. To test the function activity we used immunofluorescent analysis and cytotoxicassay for TNF-? detection. It was observed that LYVE-1+macrophages are able to bind and actively uptake hyaluronic acid. Moreover, it was shown that TNF-? production activity of LYVE-1+macrophages was significantly higher in comparison with peritoneal macrophages of rats. Thus based on our and literature data we suggest that LYVE-1+macrophages may participate in homeostasis of hyualuronic acid metabolism in subcutaneous fascia that is devoid of lymphatic vessels.
Список литературы:
1. Banerji S, Ni J, Wang S-X, et al. 1999. LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J Cell Biol. 144:789–801.
2. Prevo R, Banerji S, Ferguson D, Jackson DG. 2001. Mouse LYVE-1 is an endocytic receptor for hyaluronan in lymphatic endothelium. J Biol Chem. 276:19,420–19,430.
3. Beasley NJP, Prevo R, Banerji S, et al. 2002. Intratumoral lymphangiogenesis and lymph node metastasis in head and neck cancer. Cancer Res. 62:1315–1320.
4. Carreira CM, Nasser SM, di Tomaso E, et al. 2001. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 61:8079–8084.
5. Grant AJ, Goddard S, Ahmed-Choudhury J, et al. 2002. Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease. Am J Pathol. 160:1445–1455.
6. Nonaka H, Tanaka M, Suzuki K, Miyajima A. 2007. Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors. Dev Dyn. 236:2258–2267.
7. Cursiefen, C., et al. 2004. VEGF-A stimulates lymphangiogenesis and hemangiogenesis in inflammatory neovascularization via macrophage recruitment. J. Clin. Invest. 113:1040–1050.
8. Maruyama K, Ii M, Cursiefen C, et al. 2005. Inflammation-induced lymphagiogenesis in the cornea arises from CD11b-positive macrophages, J Clin Invest. 115: 2363-2372.
9. Schledzewski K, et. al. 2006. Lymphatic endothelium-specific hyaluronan receptor LYVE-1 is expressed by stabilin-1+, F4/80+, CD11b+macrophages in malignant tumours and wound healing tissue in vivo and in bone marrow cultures in vitro:implications for the assessment of lymphangiogenesis; J Pathol. 209: 67–77.
10. Heping Xu, Mei Chen, Delyth M. Reid, and John V. Forrester. 2007. LYVE-1–Positive Macrophages Are Present in Normal Murine Eyes. 48:2162-2171.
11. Bockle BC, Solder E, Kind S, Romani N, Sepp N.T. 2008, DC-SIGN+CD163+macrophages expressing hyaluronan receptor LYVE-1 are located within chorion of the placenta. Placenta. 29:187-192.
12. Schroedl F, Brehmer A, Neuhuber WL, Kruse FE, May CA, Cursiefen C. 2008. The normal human choroids is endowed with a significant number of lymphatic vessel endothelial hyaluronate receptor 1(LYVE-1)-positive macrophages. IVOS.49(12):5222-9.
13. Ogay V, Soh K.S. 2008. Expression of lymphatic vessel endothelial hyaluronan receptor-1 on macrophages in rat subcutaneous connective tissue. FASEB J. 22:1198,6.
14. Rosental GJ, Corsini E. 1995. Tumor necrosis factor-? in immunotoxicity assessment. Methods in Immunotoxicology. 327-343.
15. Jackson DG. 2003. The lymphatic revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 13:1-7.
16. Tripathi RC, Tripathi BJ. 1982. Human trabecular endothelium, corneal endothelium, keratocytes, and scleral fibroblasts in primary cell culture: a comparative study of growth characteristics, morphology,and phagocytic activity by light and scanning electron microscopy. Exp Eye Res. 35:611–624.
17. Laurent TC, Fraser JR.1992. Hyaluronan. FASEB J. 6:2397–2404.
18. Reed RK, Laurent UB. 1992. Turnover of hyaluronan in the microcirculation. Am Rev Respir Dis. 146:S37–S39.
19. Maruyama K, Asai J, Li M, Thorne T, Losordo DW, D’ Amore PA. 2007. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing. Am J Pathol. 170:1178-1191.
1. Banerji S, Ni J, Wang S-X, et al. 1999. LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J Cell Biol. 144:789–801.
2. Prevo R, Banerji S, Ferguson D, Jackson DG. 2001. Mouse LYVE-1 is an endocytic receptor for hyaluronan in lymphatic endothelium. J Biol Chem. 276:19,420–19,430.
3. Beasley NJP, Prevo R, Banerji S, et al. 2002. Intratumoral lymphangiogenesis and lymph node metastasis in head and neck cancer. Cancer Res. 62:1315–1320.
4. Carreira CM, Nasser SM, di Tomaso E, et al. 2001. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 61:8079–8084.
5. Grant AJ, Goddard S, Ahmed-Choudhury J, et al. 2002. Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease. Am J Pathol. 160:1445–1455.
6. Nonaka H, Tanaka M, Suzuki K, Miyajima A. 2007. Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors. Dev Dyn. 236:2258–2267.
7. Cursiefen, C., et al. 2004. VEGF-A stimulates lymphangiogenesis and hemangiogenesis in inflammatory neovascularization via macrophage recruitment. J. Clin. Invest. 113:1040–1050.
8. Maruyama K, Ii M, Cursiefen C, et al. 2005. Inflammation-induced lymphagiogenesis in the cornea arises from CD11b-positive macrophages, J Clin Invest. 115: 2363-2372.
9. Schledzewski K, et. al. 2006. Lymphatic endothelium-specific hyaluronan receptor LYVE-1 is expressed by stabilin-1+, F4/80+, CD11b+macrophages in malignant tumours and wound healing tissue in vivo and in bone marrow cultures in vitro:implications for the assessment of lymphangiogenesis; J Pathol. 209: 67–77.
10. Heping Xu, Mei Chen, Delyth M. Reid, and John V. Forrester. 2007. LYVE-1–Positive Macrophages Are Present in Normal Murine Eyes. 48:2162-2171.
11. Bockle BC, Solder E, Kind S, Romani N, Sepp N.T. 2008, DC-SIGN+CD163+macrophages expressing hyaluronan receptor LYVE-1 are located within chorion of the placenta. Placenta. 29:187-192.
12. Schroedl F, Brehmer A, Neuhuber WL, Kruse FE, May CA, Cursiefen C. 2008. The normal human choroids is endowed with a significant number of lymphatic vessel endothelial hyaluronate receptor 1(LYVE-1)-positive macrophages. IVOS.49(12):5222-9.
13. Ogay V, Soh K.S. 2008. Expression of lymphatic vessel endothelial hyaluronan receptor-1 on macrophages in rat subcutaneous connective tissue. FASEB J. 22:1198,6.
14. Rosental GJ, Corsini E. 1995. Tumor necrosis factor-? in immunotoxicity assessment. Methods in Immunotoxicology. 327-343.
15. Jackson DG. 2003. The lymphatic revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 13:1-7.
16. Tripathi RC, Tripathi BJ. 1982. Human trabecular endothelium, corneal endothelium, keratocytes, and scleral fibroblasts in primary cell culture: a comparative study of growth characteristics, morphology,and phagocytic activity by light and scanning electron microscopy. Exp Eye Res. 35:611–624.
17. Laurent TC, Fraser JR.1992. Hyaluronan. FASEB J. 6:2397–2404.
18. Reed RK, Laurent UB. 1992. Turnover of hyaluronan in the microcirculation. Am Rev Respir Dis. 146:S37–S39.
19. Maruyama K, Asai J, Li M, Thorne T, Losordo DW, D’ Amore PA. 2007. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing. Am J Pathol. 170:1178-1191.
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Бұл жұмыста тері асты шандырларынан бөлініп алынған LYVE-1+ макрофагтарының функционалдық белсенділігін анықтау үшін иммунофлуоресцентті талдау және ІНФ-? (ФНО-?) анықтау үшін цитотоксикалықтест пайдаланылды. LYVE-1+макрофагтардың гиалуронан қышқылын байланыстыру және оны сіңіру қасиетіне ие екені байқалды. Сонымен қатар LYVE-1+ макрофагтарының ІНФ-? өндіруші белсенділігіперитонеалдық қуыс макрофагтарына қарағанда жоғары. Әдебиеттер және біздің мәліметтер бойынша LYVE-1+ макрофагтар лимфалық тамырлары жоқ егеуқұйрық тері асты шандырларының гиалурон қышқылыметаболизміне, гомеостазына қатысады.
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